廣州市碩恒生物科技有限公司是廣州知名的試劑供應商,產品涉及分子實驗,蛋白組學,細胞培養(yǎng),微生物培養(yǎng),植物組培,生化實驗,耗材,儀器等,主要代理的品牌有:Sigma,Invitrogen (Gibco)、abcam 、Merckmillipore、Roche ,Qaigen、GE、BD、NEB,南京建成、R D、Peprotech、CST、Santa Cruz 、北京康為世紀、Pharmacia、Thermo, Wako、Whatman、CeneCopoeia、Clontech、Omega,Promega,KPL、天恩澤,Amresco等。耗材類主要有:NUNC,Axygen、Corning。
品牌:Sciencell是否是腫瘤細胞:0運輸方式:干冰年限:長期數(shù)量:大量規(guī)格:5 x 10^5 cells/vial
人臍靜脈內皮細胞說明:
貨號:8000
血管內皮細胞對維持血管動態(tài)平衡起著重要作用。它們合成、分泌凝血和纖溶系統(tǒng)的激活因子和抑制因子、影響血小板粘附和聚集的調節(jié)因子。內皮細胞還釋放控制細胞增殖和調節(jié)血管壁緊張度的分子。這些過程都可以用培養(yǎng)的細胞進行體外研究,人臍靜脈和動脈內皮細胞是這類研究中zui常用的細胞類型。除了普通的內皮細胞特征例如“鵝卵石狀”,VIII因子陽性著色,能攝取乙?;兔芏戎鞍?,人臍血管內皮細CD31染色陽性。用IL-1或TNF-α預先處理過的細胞也能選擇性地表達E-selectin和VCAM。 人臍靜脈內皮細胞(HUVEC)提取于人臍帶靜脈組織,原代凍存。每管含有細胞數(shù) 5×105 cells/ml,此細胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗證,經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。
推薦培養(yǎng)基: (ECM, Cat. No. 1001)
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood plaets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the most commonly used cell type for such studies. Except for the common endothelial cell features such as "cobblestone" morphology, positive staining for Factors VIII and the ability to take up acetylated low-density lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] and VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are cryopreserved at passage one and delivered frozen. Each vial contains 5 x 105 cells in 1 ml volume. HUVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In "Human Cell Culture Protocols" (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
Sciencell產品目錄:
胎盤 細胞系統(tǒng)中文名貨號簡稱英文名稱人羊膜上皮細胞7110HAEpiCHuman Amniotic Epithelial Cells人長茸毛滋養(yǎng)層7120HVTHuman Villous Trophoblasts人長茸毛間葉成纖維細胞7130HVMFHuman Villous Mesenchymal Fibroblasts 脂肪細胞系統(tǒng)中文名貨號簡稱英文名稱人脂肪微血管內皮細胞7200HAMECHuman Adipose Microvascular Endothelial Cells人脂肪前體細胞-內臟7210HPA-vHuman Preadipocytes-visceral人脂肪前體細胞-皮下7220HPA-sHuman Preadipocytes-subcutaneous 卵巢細胞系統(tǒng)中文名貨號簡稱英文名稱人卵巢微血管內皮細胞7300HOMECHuman Ovarian Microvascular Endothelial Cells人卵巢表面上皮細胞7310HOSEpiCHuman Ovarian Surface Epithelial Cells人卵巢成纖維細胞7330HOFHuman Ovarian Fibroblasts 間充干細胞系統(tǒng)中文名貨號簡稱英文名稱人羊膜間充質干細胞7140HAmMSCHuman Amniotic Mesenchymal Stromal Cells人絨毛膜間充質干細胞7150HCMSCHuman Chorionic Mesenchymal Stromal Cells人骨髓間充質干細胞7500HMSC-bmHuman Bone Marrow-derived Mesenchymal Stem Cells人脂肪間充質干細胞7510HMSC-adHuman Adipose-derived Mesenchymal Stem Cells人肝間充質干細胞7520HMSC-heHuman Liver-derived Mesenchymal Stem Cells人臍間充質干細胞7530HUMSCHuman Umbilical Mesenchymal Stem Cells人肺間充質干細胞7540HPMSCHuman Pulmonary Mesenchymal Stem Cells人脊椎間充質干細胞7550HVMSCHuman Vertebral Mesenchymal Stem Cells 乳腺細胞系統(tǒng)中文名貨號簡稱英文名稱人乳腺微血管內皮細胞7600HMMECHuman Mammary Microvascular Endothelial Cells人乳腺上皮細胞7610HMEpiCHuman Mammary Epithelial Cells人乳腺成纖維細胞7630HMFHuman Mammary Fibroblasts 干細胞誘導細胞中文名貨號簡稱英文名稱人iPS誘導心肌細胞6240HPSC-CCHPSC-derived Cardiomyocyte Cells 臍帶細胞系統(tǒng)中文名貨號簡稱英文名稱人臍靜脈內皮細胞8000HUVECHuman Umbilical Vein Endothelial Cells人臍動脈內皮細胞8010HUAECHuman Umbilical Artery Endothelial Cells人臍靜脈平滑肌細胞8020HUVSMCHuman Umbilical Vein Smooth Muscle Cells人臍動脈平滑肌細胞8030HUASMCHuman Umbillical Artery Smooth Muscle Cells 大鼠細胞系統(tǒng)中文名貨號簡稱英文名稱大鼠腦微血管內皮細胞R1000RBMECRat Brain Microvascular Endothelial Cells大鼠垂體細胞R1200RPCRat Pituitary Cells大鼠腦膜細胞R1400RMCRat Meningeal Cells大鼠腦脊神經元R1510RN-rRat Neurons-raphe大鼠皮質神經元R1520RN-cRat Neurons-cortical大鼠顆粒細胞R1530RGCRat Granule Cells大鼠神經元- 海馬R1540RN-hRat Neurons-hippocampal大鼠黑質神經元R1550RN-snRat Neurons-substantia nigra大鼠紋狀體神經元R1560RN-sRat Neurons-striatal大鼠腹脊髓神經元R1570RN-vscRat Neurons-ventral spinal cord大鼠背脊髓神經元R1580RN-dscRat Neurons-dorsal spinal cord大鼠背脊髓神經元R1590RN-scRat Neurons-spinal cord大鼠少突膠質前體細胞R1600ROPCRat Oligodendrocyte Precursor Cells大鼠雪旺細胞R1700RSCRat Schwann Cells大鼠神經束膜成纖維細胞R1710RPNFRat Perineurial Fibroblasts大鼠星形膠質細胞R1800RARat Astrocytes大鼠星形膠質細胞- 小腦R1810RA-cRat Astrocytes-cerebellar大鼠星形膠質細胞- 海馬R1820RA-hRat Astrocytes-hippocampal大鼠小神經膠質細胞R1900RMRat Microglia大鼠巨噬細胞R1920RMaRat Macrophage大鼠表皮角質形成細胞-新生大鼠R2100REK-nRat Epidermal Keratinocytes-neonate大鼠真皮成纖維細胞R2300RDF-nRat Dermal Fibroblasts大鼠真皮成纖維細胞- 成人R2320RDF-aRat Dermal Fibroblasts-adult大鼠淋巴成纖維細胞R2530RLFRat Lymphatic Fibroblasts大鼠唾液腺成纖維細胞R2670RSGFRat Salivary Gland Fibroblasts大鼠肺肺泡上皮細胞R3200RPAEpiCRat Pulmonary Alveolar Epithelial Cells大鼠肺成纖維細胞R3300RPFRat Pulmonary Fibroblasts大鼠腎小管上皮細胞R4100RRPTEpiCRat Renal Proximal Tubular Epithelial Cells大鼠腎系膜細胞R4200RRMCRat Renal Mesangial Cells大鼠肝星狀細胞R5300RHSteCRat Hepatic Slate Cells大鼠肝巨噬細胞R5340RHMaRat Hepatic Macrophage大鼠主動脈平滑肌細胞R6110RASMCRat Aortic Smooth Muscle Cells大鼠心肌細胞R6200RCMRat Cardiac Myocytes大鼠心肌成纖維細胞R6300RCFRat Cardiac Fibroblasts大鼠角膜基質細胞R6520RKRat Keratocytes大鼠晶狀體上皮細胞R6550RLEpiCRat Lens Epithelial Cells大鼠間充質干細胞-骨髓R7500RMSC-bmRat Mesenchymal Stem Cells-bone marrow大鼠胚胎成纖維細胞R7540REFRat Embryonic Fibroblasts
(三)小鼠正常細胞
中文名稱貨號簡稱英文名稱小鼠腦膜細胞M1400-57MMCMouse Meningeal Cells小鼠腦脊神經元M1510-57MN-rMouse Neurons-raphe小鼠皮質神經元M1520-57MN-cMouse Neurons-cortical小鼠顆粒細胞M1530-57MGCMouse Granule Cells小鼠海馬趾神經元M1540-57MN-hMouse Neurons-hippocampal小鼠腹脊髓神經元M1570-57MN-vscMouse Neurons-ventral spinal cord小鼠背脊髓神經元M1580-57MN-dsc]Mouse Neurons-dorsal spinal cord小鼠腹脊髓神經元M1590-57MN-vscMouse Neurons-ventral spinal cord小鼠雪旺細胞M1700-57MSCMouse Schwann Cells小鼠星形膠質細胞M1800-57MAMouse Astrocytes小鼠小腦星形膠質細胞M1810-57MA-cMouse Astrocytes-cerebellar小鼠海馬趾星形膠質細胞M1820-57MA-hMouse Astrocytes-hippocampal小鼠脊髓星形膠質細胞M1830-57MA-sc Mouse Astrocytes-spinal cord小鼠小膠質細胞M1900-57MMMouse Microglia小鼠巨噬細胞M1920-57MMa-bmMouse Macrophage小鼠淋巴成纖維細胞M2530-57MLFMouse Lymphatic Fibroblasts小鼠唾腺成纖維細胞M2670-57MSGF Mouse Salivary Gland Fibroblasts小鼠腎小管上皮細胞M4100-57MRTEpiCMouse Renal Tubular Epithelial Cells小鼠心肌細胞M6200-57MCMMouse Cardiac Myocytes小鼠心臟成纖維細胞M6300-57MCFMouse Cardiac Fibroblasts小鼠骨髓間充質干細胞M7500-57MMSC-bmMouse Mesenchymal Stem Cells-bone marrow小鼠胚胎成纖維細胞M7540-57MEF-mtMouse Embryonic Fibroblasts(四)豬細胞系統(tǒng)豬視網膜星形膠質細胞P1870PRAPorcine Retinal Astrocytes 豬角膜上皮細胞P6510PCEpiCPorcine Corneal Epithelial Cells豬視網膜色素上皮細胞P6540PRPEpiCPorcine Retinal Pigment Epithelial Cells 豬晶狀體上皮細胞P6550PLEpiCPorcine Lens Epithelial Cells (五)馬和狗細胞系統(tǒng)
馬間充質干細胞--脂肪H7510HMSC-adHorse Mesenchymal Stem Cells-adipose馬間充質干細胞--脂肪E7510EMSC-adEquine Mesenchymal Stem Cells-adipose狗間充質干細胞--脂肪D7510DMSC-adDog Mesenchymal Stem Cells-adipose
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