ZYMO貨號(hào)D5005 EZ DNA Methylation-Gold Kit 將DNA變性與亞硫酸氫鹽轉(zhuǎn)化過程變?yōu)橐徊剑瞬襟E采用溫度變性來取代先前操作步驟中使用氫氧化鈉的化學(xué)變性，經(jīng)過亞硫酸氫鹽處理后試劑盒可大量回收到DNA。該試劑盒是基于胞嘧***與亞硫酸氫鹽之間發(fā)生的使胞嘧***轉(zhuǎn)化為尿嘧***的三步反應(yīng)過程。ZYMO貨號(hào)D5005 EZ DNA Methylation-Gold Kit 采用創(chuàng)新的柱內(nèi)脫磺酸技術(shù)來減少不必要的DNA沉淀，以便提供給研究人員更好的結(jié)果。試劑盒的設(shè)計(jì)可以減少模板降解和純化過程中的DNA損失，并且完全轉(zhuǎn)化未甲基化的胞嘧***?；厥盏腄NA對(duì)于PCR、、測(cè)序、微陣列等下游分析是很理想的。整個(gè)過程在3小時(shí)內(nèi)完成。

ZYMO EZ DNA Methylation-Gold Kit優(yōu)勢(shì)

（1）時(shí)間 全部操作可在3小時(shí)內(nèi)完成

（2）轉(zhuǎn)化效率 完全轉(zhuǎn)化富含GC的DNA

（3）DNA回收效率 與特殊配制的轉(zhuǎn)化緩沖液一起發(fā)揮作用的熱變性可使在轉(zhuǎn)化過程中zui大限度的回收的DNA

 ZYMO貨號(hào)D5005 EZ DNA Methylation-Gold Kit 在3小時(shí)內(nèi)完全轉(zhuǎn)化富含GC的DNA

DNA中的胞嘧***甲基化檢測(cè)使用快速的溫度變性步驟來取代其他試劑盒所采用的耗時(shí)的化學(xué)變性步驟。

相互緊密的DNA變性與亞硫酸氫鹽處理步驟可使未甲基化的胞嘧***完全轉(zhuǎn)化為尿嘧***的過程更為流暢。

通過創(chuàng)新的spin-column技術(shù)，可一步完成DNA的純化與脫硫，并且無DNA沉淀。

 每次處理：500pg-2ug DNA

理想數(shù)值為：200-500ng
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表觀遺傳系列

產(chǎn)品名稱（Product name）貨號(hào)（Cat. No.）規(guī)格（Size）EZ DNA Methylation Kit D5001
D5002
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2ｘ96(shallow)
2ｘ96(deep)EZ DNA Methylation-Gold Kit D5005s
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D5007
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