G-5 Supplement is a chemically defined, serum-free supplement based on Bottenstein s G-5 formulation. G-5 Supplement is intended for use with Neurobasal media.
G-5 Supplement is recommended for growth and expression of glial cells （normal and tumor） of astrocytic phenotype （astrocytes）. G-5 Supplement can be used as a substitute for Bottenstein s G-2 formulation. G-5 Supplement is provided as a 100X concentrate.

Technologies Corporation （http://www.lifetechnologies.com/home.html） （Nasdaq: LIFE） 是一家致力于改善人類生存環(huán)境的全球性生物技術(shù)公司。該公司的儀器、耗材和服務(wù)可協(xié)助研究人員加快推進(jìn)科學(xué)和醫(yī)學(xué)的發(fā)展，從而讓人們的生活變得更加美好。該公司的客戶遍及生物學(xué)各個領(lǐng)域，包括篩選與轉(zhuǎn)化研究、分子藥物、干細(xì)胞治療、食品安全和動物保健以及21世紀(jì)的法醫(yī)鑒定等。該公司生產(chǎn)供分子診斷和僅供研究使用的產(chǎn)品。Life Technologies的業(yè)界ling先品牌，包括創(chuàng)新型Applied Biosystems和Ion Torrent品牌儀器以及Invitrogen、Gibco、Ambion、Molecular Probes和Taqman等被全球各地的生命科學(xué)實驗室所廣泛使用。該公司2010年的銷售額為36億美元，在全球160多個國家和地區(qū)擁有員工約11,000人，公司持有將近3900項知識產(chǎn)權(quán)及專有許可證，堪稱生命科學(xué)界zui大規(guī)模的知識產(chǎn)權(quán)資產(chǎn)?；A(chǔ)培養(yǎng)基,細(xì)胞培養(yǎng)基,細(xì)胞培養(yǎng)

產(chǎn)品包括細(xì)胞培養(yǎng)基，動物血清，細(xì)胞培養(yǎng)常用試劑，分子生物學(xué)領(lǐng)域試劑。

細(xì)胞培養(yǎng)       特級胎牛血清（北美血源） GIBCO 16000-044 500ml 特級胎牛血清（澳洲血源） GIBCO 10099-141 500ml 優(yōu)級胎牛血清 PAA 15-101 500ml 普通型胎牛血清（南美血源） Hyclone  SV30087.02 500ml 特級胎牛血清（澳洲血源） Hyclone  SH30084.03 500ml 標(biāo)準(zhǔn)型胎牛血清（澳大利亞來源） Hyclone  SH30370.03 500ml 特級胎牛血清（北美血源） Hyclone  SH30070.03 500ml 胎牛血清  烏拉圭 GIBCO C2027050  500ml MSC QUALIFIED FBS  GIBCO 12662029 500ml FBS, HI  GIBCO 10438026 500ml FBS, ES CELL QUAL. USDA APPD.  GIBCO 10439024 500ml FBS, DIALYZED, USA ORIGIN  GIBCO 26400044 500ml FBS QUALIFIED, USA ORIGIN  GIBCO 26140079 500ml FBS, ES CELL QUAL, USA ORIGIN  GIBCO 16141079 500ml FBS, HI  GIBCO 16140071 500ml 巴西胎牛 GIBCO 12657029 500ml 新生牛血清 GIBCO 16010159 500ml FOETAL BOVINE SERUM Hyclone  SH30406.02 500ml New Zealand Calf Serum Hyclone  SH30401.01 500ml FetalClone II Hyclone  SH30066.02 100ml STANDARD FETAL BOVINE SERUM Hyclone  SH30088.03 500ml Newborn Bovine Calf Serum, U.S. Origin Hyclone  SH30118.02 100ml FETAL BOVINE SERUM Hyclone  SH30070.02 100ml COSMIC CALF SERUM Hyclone  SH30087.02 100ml FETALBOVINESERUM CHARACTERIZSED Hyclone  SH30070.03E 500ml FetalClone I Hyclone  SH30080.03 500ml FETAL BOVINE SERUM CHARACTERIZED Hyclone  SH30396.03 500ml FETAL BOVINE SERUM Hyclone  SH30071.03 500ml FETAL BOVINE SERUM Hyclone  SH30071.02 100ml FetalClone I Hyclone  SH30080.02 100ml FETAL BOVINE SERUM CHARACTERIZSED Hyclone  SH30070.02E 100ml FETAL BOVINE SERUM Hyclone  SH30079.02 100ml CHARCOAL/DEXTRAN TREATED FBS Hyclone  SH30068.02 100ml CHARCOAL/DEXTRAN TREATED FBS Hyclone  SH30068.03 500ml FetalClone II Hyclone  SH30066.03 500ml HyClone FetalClone III Hyclone  SH30109.03 500ml STANDARD FETAL BOVINE SERUM Hyclone  SH30088.03 500ml STANDARD FETAL BOVINE SERUM Hyclone  SH30088.02 100ml FETAL BOVINE SERUM Hyclone  SH30079.03 500ml 馬血清 Hyclone  SH30074.03 500ml 1640培養(yǎng)基 1640 Medium GIBCO 31800-022 10X1L DMEM（高糖）含丙酮酸鈉 GIBCO 12800-017 10X1L DMEM（高糖） DMEM（High Glucose） GIBCO 12100-046 10X1L DMEM（低糖）DMEM（Low Glucose） GIBCO 31600-034 10X1L MEM 培養(yǎng)基 GIBCO 41500-034 10X1L M199培養(yǎng)基 M199 Medium GIBCO 31100-035 10X1L F12培養(yǎng)基  GIBCO 21700-075 10X1L PBS Gibco 10010023 500ml/瓶 PBS- PH 7.4 Invitrogen 10010049 10x500ML EXPRESS FIVE SFM invitrogen 10486025 1000ML DNAZOL REAGENT invitrogen 10503027 100ML SF 900 II SFM Invitrogen 10902-096 500ML OPTI MEM I invitrogen 11058021 500ML HT SUP- 100X Invitrogen 11067030 50ML MEM VIT SOL invitrogen 11120052 100ML MEM AMINO ACIDS SOL- 50X Invitrogen 11130-051 100ML MEM NEAA- 100X Invitrogen 11140-050 100ML GRACES INSECT MED GIBCO 11300027 10X1L ADVANCED D-MEM Invitrogen 12491015 500ml HBSS invitrogen 14025126 6 X 1000ML DPBS Invitrogen 14040141 100ML- PLASTIC- E-Z HOLD HBSS invitrogen 14175-095 500ml HBSS Invitrogen 14175145 6 X 1000ML DPBS 1x w/o Ca and Mg Gibco 14190-144 500ML  D GLUCOSE invitrogen 15023021 1KG PENICILLIN STREPTOMYCIN SOL GIBCO 15070063 100ml TRYPSIN- 2.5%- 10X Gibco 15090046 100ml  PENICILLIN STREPTOMYCIN SOL 青鏈霉素 invitrogen 15140122 100ML ANTIBIOTIC ANTIMYCOTIC invitrogen 15240062 100ml ANTIBIOTIC ANTIMYCOTIC invitrogen 15240096 20ML HUMAN COT-1 DNA invitrogen 15279011 500 UG LIPOFECTAMINE LTX- 1ML invitrogen 15338100 1ml YEAST TRNA Invitrogen 15401011 25 MG TRIZOL REAGENT invitrogen 15596026 100ML TRlzol Reagent Gibco 15596018 200ml NBCS invitrogen 16010159 500ML RABBIT SERUM Invitrogen 16120099 100ML- PLASTIC- E-Z HOLD Fetal Bovine Serum, Qualified, Heat-Inactivated  Invitrogen 16140-063 100 ml COLLAGENASE- TYPE I  Gibco 17100017 1G COLLAGENASE- TYPE II GIBCO 17101015 1g CO2 INDEPENDENT MED Invitrogen 18045088 500ML BIOPRIME CGH LABELING SYSTEM.. invitrogen 18095-011 30 REACTIONS YEASTOLATE- 50X- GIBCO 18200048 100ml PBS- PH 7.2  Gibco 20012050 10x500mL Neurbasal Medium Gibco 21103049 500ml  F 12 NUTRIENT MIX- KAIGHNS MOD Invitrogen 21127022 500ML- PLASTIC- E-Z HOLD dpbs Invitrogen 21600010 10X1L F 12 NUTRIENT MIX- HAMS Gibco 21700075 10X1L 2 MERCAPTOETHANOL 1000X Invitrogen 21985023 50ml RPMI 1640 gibco 22400089 500mL- PLASTIC- E-Z HOLD RPMI 1640 invitrogen 23400021 10X1L PLURonIC invitrogen 24040032 100ML- PLASTIC- E-Z HOLD L GLUTAMINE- 100X Invitrogen 25030149 20ML TRYPSIN 0.25% EDTA invitrogen 25200056 100ML  TRYPSIN 0.25% EDTA（0.25%的胰酶，含EDTA 也含酚紅） gibco 25200072 500ML TRYPSIN .05% EDTA invitrogen 25300054 100ml TRYPSIN .05% EDTA invitrogen 25300062 500ml SELECT AGAR invitrogen 30391023 500G OPTI MEM I invitrogen 31985062 100ML- PLASTIC- E-Z HOLD opti-MEM reduce serum medium375 invitrogen 31985070 500ml INSULIN-TRANS-SEL-G- 100X Invitrogen 41400045 10ml DMEM NUTRIENT MIX F12 Invitrogen-Gibco 11330032 500ml SODIUM PYRUVATE SOL  Gibco 11360070 100mL  LIPOFECTAMINE 2001 REAGENT Gibco 11668027 0.75ml GATEWAY LR CLonASE ENZYME MIX.. invitrogen 11791019 20 REACTIONS GENETICIN invitrogen 11811031 5G RPMI 1640 invitrogen 11875119 10x500ML  DMEM lo gluc w/glut Gibco 11885-084 500ML  MEM ALPHA Invitrogen 11900024 10X1L DMEM gibco 11960044 500ML- PLASTIC- E-Z HOLD DMEM Invitrogen-Gibco 11965084 1000ml Dulbecco's Modified Eagle Medium （DMEM） （1X）, liquid （high glucose）  invitrogen 11995-065 500ML- PLASTIC- E-Z HOLD MEM ALPHA Invitrogen 12000022 10X1L ELECTROMAX DH10B T1 CELLS invitrogen 12033015 0.5ml HYBRIDOMA SFM Gibco 12045076 1000ML- PLASTIC- E-Z HOLD oligofectamine reagent Invitrogen 12252011 1ml IMDM invitrogen 12440-053 500ml DMEM/F12培養(yǎng)基 GIBCO 12400-024 10X1L IMDM培養(yǎng)基 GIBCO 12200-036 10X1L DMEM（高糖）培養(yǎng)液 Hyclone SH30022.01B 500ml DMEM（低糖） 培養(yǎng)液 Hyclone SH30021.01B 500ml 1640培養(yǎng)液 Hyclone SH30809.01B 500ml DMEM/F12培養(yǎng)液 Hyclone SH30023.01B 500ml -MEM培養(yǎng)液 Hyclone SH30265.01B 500ml MEM/EBSS培養(yǎng)液 Hyclone SH30265.01B 500ml 胰蛋白酶/EDTA消化液 Hyclone SV30042.01 100ml 青/鏈霉素濃縮液 Hyclone SV30010 100ml Lipofectamine 2000 Transfection Reagent
 脂質(zhì)體2000 GIBCO 11668-027 0.75ml Lipofectamine 2000 Transfection Reagent
 脂質(zhì)體2000 GIBCO 11668-019 1.5ml B-27 Serum-Free Supplement （50X）, Liquid 
  GIBCO 17504-044 10ml N-2 Supplement （100X）, Liquid
  GIBCO 17502-048 5ml G-5 Supplement （100X）, Liquid  GIBCO 17503-012  1ml SUPERscript II invitrogen 18064014   SUPERscript III invitrogen 18080044   SUPERscript III 1ST invitrogen 18080051   Wizard SV Gel and PCR Clean-Up System promega A9281 50 preps Wizard SV Gel and PCR Clean-Up System promega A9282 250 preps Wizard SV Gel and PCR Clean-Up System promega A9285 1000 preps Dual-Glo Luciferase Assay System promega E2920 10ml Dual-Glo Luciferase Assay System promega E2940 100ml Dual-Glo Luciferase Assay System promega E2980 10 100ml Dual-Luciferase Reporter Assay System promega E1910 100 assays Dual-Luciferase Reporter Assay System 10-Pack promega E1960 1,000 assays Dual-Luciferase Reporter 1000 Assay System promega E1980 1,000 assays ImProm-II Reverse Transcriptase promega A3803 500 reactions ImProm-II Reverse Transcription System promega A3800 100 reactions Reverse Transcription System promega A3500 100 reactions CellTiter 96 Non-Radioactive Cell Proliferation Assay promega G4000 1,000 assays CellTiter 96 Non-Radioactive Cell Proliferation Assay promega G4100 5,000 assays CellTiter 96 AQueous One Solution Cell Proliferation Assay promega G3580 1,000 assays CellTiter 96 AQueous One Solution Cell Proliferation Assay promega G3581 5,000 assays CellTiter 96 AQueous One Solution Cell Proliferation Assay promega G3582 200 assays CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay promega G5421 1,000 assays CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay promega G5430 5,000 assays CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay promega G5440 50,000 assays pGEM -T Easy Vector System I promega A1360 20 reactions pGEM -T Easy Vector System II promega A1380 20 reactions DeadEnd Fluorometric TUNEL System promega G3250 60 reactions DeadEnd Colorimetric TUNEL System promega G7130 40 reactions DeadEnd Colorimetric TUNEL System promega G7360 20 reactions M-MLV Reverse Transcriptase promega M1701 10,000u M-MLV Reverse Transcriptase promega M1705 50,000u GoTaq qPCR Master Mix, promega A6001 200 Reactions GoTaq qPCR Master Mix, promega A6002 1000 Reactions Wizard DNA Clean-Up System promega A7280 100 preps SV Total RNA Isolation System promega Z3100 50 preps SV Total RNA Isolation System promega Z3101 10 preps SV Total RNA Isolation System promega Z3105 250 preps CellTiter-Glo Luminescent Cell Viability Assay promega G7570 10ml CellTiter-Glo Luminescent Cell Viability Assay promega G7571 10 10ml CellTiter-Glo Luminescent Cell Viability Assay promega G7572 100ml CellTiter-Glo Luminescent Cell Viability Assay promega G7573 10 100ml PepTag Non-Radioactive PKC Assay promega V5330 120 reactions PepTag Non-Radioactive cAMP-Dependent Protein Kinase Assay promega V5340 120 reactions