亚洲欧美日本韩国_久久久久亚洲AV片无码V_亚洲AV片不卡无码一_H漫全彩纯肉无码网站

 
 
當前位置: 首頁 » 新聞資訊 » 廠商 » 正文

CRL-1420-MIAPaCa-2 胰腺癌細胞系_腫瘤細胞 人類腫瘤細胞系目錄-上海桑戈生物科技有限公司

分享到:
放大字體  縮小字體    發(fā)布日期:2019-09-02  來源:儀器信息網  作者:Mr liao  瀏覽次數:358
核心提示:CRL-1420 MIAPaCa-2 胰腺癌細胞系ATCC Number: CRL-1420 Designations: MIA PaCa-2 Depositors: A Yunis Biosafety Level: 1 Shipped: frozen Medium Serum: See Propagation RL-1420 MIAPaCa-2 胰腺癌細胞系ATCC Number:CRL-1420 Designations:MIA PaCa-2Depositors:A YunisBiosafety Le
CRL-1420 MIAPaCa-2 胰腺癌細胞系
ATCC Number: CRL-1420
Designations: MIA PaCa-2
Depositors: A Yunis
Biosafety Level: 1
Shipped: frozen
Medium Serum: See Propagation

RL-1420 MIAPaCa-2 胰腺癌細胞系

ATCC  Number:CRL-1420  Designations:MIA PaCa-2Depositors:A YunisBiosafety Level:1Shipped:frozenMedium Serum:See PropagationGrowth Properties:adherent, single cells and loosely attached clustersOrganism:Homo sapiens (human)Morphology:attached epithelial with floating rounded cells
 Source:Organ: pancreas 
Disease: carcinomaCellular Products:human colony stimulating factor, subclass I (CSF-I); plasminogen activatorPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.RL-1420 MIAPaCa-2 胰腺癌細胞系Isolation:Isolation date: 1975DNA Profile (STR):Amelogenin: X 
CSF1PO: 10 
D13S317: 12,13 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 12,13 
THO1: 9,10 
TPOX: 9 
vWA: 15Cytogenetic Analysis:This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.Isoenzymes:G6PD, BAge:65 yearsGender:maleEthnicity:CaucasianComments:The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034] 
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034] 
Hybridomas have been made against the CSF-I produced by this line ATCCHB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). 
The line is sensitive to asparaginase.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco s Modified Eagle s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0 CSubculturing:Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040References:1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
58034: Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918ATCC  Number:CRL-1420 Price: Designations:MIA PaCa-2Depositors:A YunisBiosafety Level:1Shipped:frozenMedium Serum:See PropagationGrowth Properties:adherent, single cells and loosely attached clustersOrganism:Homo sapiens (human)Morphology:attached epithelial with floating rounded cells
 Source:Organ: pancreas 
Disease: carcinomaCellular Products:human colony stimulating factor, subclass I (CSF-I); plasminogen activatorPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Isolation:Isolation date: 1975DNA Profile (STR):Amelogenin: X 
CSF1PO: 10 
D13S317: 12,13 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 12,13 
THO1: 9,10 
TPOX: 9 
vWA: 15Cytogenetic Analysis:This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.Isoenzymes:G6PD, BAge:65 yearsGender:maleEthnicity:CaucasianComments:The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034] 
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034] 
Hybridomas have been made against the CSF-I produced by this line ATCCHB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). 
The line is sensitive to asparaginase.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco s Modified Eagle s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0 CSubculturing:Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040References:1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
58034: Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918

ATCC Number: CRL-1420 Price: $279.00 
Designations: MIA PaCa-2 
Depositors: A Yunis 
Biosafety Level: 1 
Shipped: frozen 
Medium Serum: See Propagation 
Growth Properties: adherent, single cells and loosely attached clusters 
Organism: Homo sapiens (human) 
Morphology: attached epithelial with floating rounded cells


Source: Organ: pancreas 
Disease: carcinoma 
Cellular Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 

Isolation: Isolation date: 1975 
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10 
D13S317: 12,13 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 12,13 
THO1: 9,10 
TPOX: 9 
vWA: 15 
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent. 
Isoenzymes: G6PD, B 
Age: 65 years 
Gender: male 
Ethnicity: Caucasian 
Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034] 
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034] 
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). 
The line is sensitive to asparaginase. 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco s Modified Eagle s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0 C 
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040 
References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455CRL-1420 ,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和zui優(yōu)培養(yǎng)條件!

RL-1420

RL-1420

RL-1420

 
 
打賞
[ 新聞資訊搜索 ]  [ 加入收藏 ]  [ 告訴好友 ]  [ 打印本文 ]  [ 違規(guī)舉報 ]  [ 關閉窗口 ]
免責聲明:
本網站部分內容來源于合作媒體、企業(yè)機構、網友提供和互聯網的公開資料等,僅供參考。本網站對站內所有資訊的內容、觀點保持中立,不對內容的準確性、可靠性或完整性提供任何明示或暗示的保證。如果有侵權等問題,請及時聯系我們,我們將在收到通知后第一時間妥善處理該部分內容。
 

CRL-1420-MIAPaCa-2 胰腺癌細胞系_腫瘤細胞 人類腫瘤細胞系目錄-上海桑戈生物科技有限公司二維碼

掃掃二維碼用手機關注本條新聞報道也可關注本站官方微信賬號:"xxxxx",每日獲得互聯網最前沿資訊,熱點產品深度分析!
 

 
0相關評論

 
久人人爽人人爽人人片av| 东北妇女肥胖bbwbbwbbw| 成年性生交大片免费看| 国产a级毛片久久久精品毛片| 日韩不卡手机视频在线观看| 国产精欧美一区二区三区| 免费无码又爽又刺激高潮视频| 亚洲av成人无码网站| 欧美黑人又粗又大高潮喷水| 爱情岛论坛网亚洲品质| 无码人妻少妇久久中文字幕| 色天使亚洲综合一区二区| 夜色福利站www国产在线视频| 欧美黑人又粗又大高潮喷水| 东京热男人av天堂| 免费看又黄又无码的网站| 精品久久久无码人妻字幂| 欧美精品国产制服第一页| 亚洲色精品vr一区二区三区| 国产无套内射又大又猛又粗又爽| 国产精品国产免费无码专区不卡| 日韩av无码精品人妻系列| 五月色丁香婷婷网蜜臀av| 大地资源高清在线视频播放| 东京热久久综合久久88| 亚洲成av人片在www| 尤物网址在线观看| 久久婷婷成人综合色| 国产精品.xx视频.xxtv| 两个黑人大战嫩白金发美女| 人人澡人人曰人人摸看| 超碰人人模人人爽人人喊手机版| 亚洲综合图色40p| 丰满少妇被粗大猛烈进人高清| 轻点好疼好大好爽视频| 成人国内精品久久久久一区| 亚洲精品综合一区二区| 色婷婷日日躁夜夜躁| 国产小呦泬泬99精品| 国产在线第一区二区三区| 99久久婷婷国产综合精品青草免费 |