B-27 添加劑是無血清添加劑，用于海馬神經元和其他中樞神經系統(tǒng)（CNS）神經元的生長和保持其短期或長期活性。B-27 添加劑是50X 的液體，可以與Neurobasal 培養(yǎng)基或Neurobasal -A 培養(yǎng)基組合使用，用于神經細胞培養(yǎng)而不需要再添加飼養(yǎng)層細胞。

 

描述B-27 添加劑是無血清添加劑，用于海馬神經元和其他中樞神經系統(tǒng)（CNS）神經元的生長和保持其短期或長期活性。B-27 添加劑是50X 的液體，可以與Neurobasal 培養(yǎng)基或Neurobasal -A 培養(yǎng)基組合使用，用于神經細胞培養(yǎng)而不需要再添加飼養(yǎng)層細胞。

B-27 添加劑適和應用于大多數神經細胞培養(yǎng)。對于特別的應用，我們提供 其他配方的B-27 添加劑.

質量控制檢測 
B-27 添加劑 進行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海馬神經元生長檢測。 通過了細菌和真菌的檢測. B-27 也做了1X 濃度的內毒素水平測試。 

僅供研究使用。除非另有說明，不得用于任何動物或人類治療或診斷。詳細說明常用規(guī)格 Cell Type:

Neuronal Cells 

Product Size:

10 ml 

Classification:

Serum-Free 

Other Additives:

Vitamin A, 

Antioxidant Cocktail, 

Insulin 

Purity or Quality Grade:

Cell Culture Grade 

Green Features:

Sustainable packaging 

Shipping Condition:

Dry Ice 

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures. 

引文和參考文獻 （17） D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels. 作者Congar P; Bergevin A; Trudeau LE雜志J Neurophysiol （2002） 87:1046-1102產品用途Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）. ID:PN64425 Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice. 作者Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;雜志Neuron （2002） 33:789-804產品用途Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37 C in a 5% CO2 humidified incubator. ID:PN47418 Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells. 作者Kabos Peter; Kabosova Andrea; Neuman Toomas;雜志J Biol Chem （2002） 277:8763-8769產品用途Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）  ID:PN63471 Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. 作者Lesuisse Christian; Martin Lee J;雜志J Neurobiol （2002） 51:9-23產品用途A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. ID:PN60461 An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells. 作者Joseph-Bravo Patricia; P rez-Mart nez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;雜志Brain Res Brain Res Protoc （2002） 9:93-104產品用途Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties ID:PN62028更多..回到頁首 按細胞系排列的引文 細胞系名稱 有機體類型 組織 SH-Sy5Y Human Brain Fetal neurons Rat Brain Neurons （post-natal/adult） Rat Brain Neurons （superior cervical ganglia - SCG） Rat Brain Neurons （cortical） Mouse Other Neural Stem Cells Rat Brain  主營：生化試劑、抗體、重組蛋白、細胞生物、分子生物、蛋白研究、微生物、Elisa試劑盒、耗材、標準品、 標準溶液 abcam、ABI、Axygen、corning、信號轉導抗體、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R D、Roche、santa、sigma、Thermo等進口品牌 以下為本公司常備現貨產品

 

*部分 invitrogen 暢銷原裝產品

 

 

品牌 貨號 名稱 規(guī)格 invitrogen 13778-075 Lipofectamine RNAiMAX 0.75ml invitrogen 13778-150 Lipofectamine RNAiMAX 1.5ml invitrogen 11668-027 Lipofectamine 2000 Reagent 0.75ml invitrogen 11668-019 Lipofectamine 2000 Reagent 1.5ml invitrogen 15596-026 TRlzol  Reagent 100ml invitrogen 15596-018 TRlzol  Reagent 200ml invitrogen 10296-010 Trizol   LS Reagent 100ml invitrogen 10296-028 Trizol   LS Reagent 200ml invitrogen 17504-044 B-27（黃顏色） 10ml invitrogen 12587-010 B-27（黃顏色）不含維生素A 10ml invitrogen 17502-048 N-2 （粉紅顏色） 5ml invitrogen 18064-014 Superscript  II Reverse Transcriptase 10,000U invitrogen 18080-044 Superscript  III Reverse Transcriptase 10,000U

 

 

第二部分 HyClone胎牛血清

 

 

品牌 貨號 名稱 規(guī)格 HyClone SH30370.03 Australian Standard Fetal Bovine Serum 500ml HyClone SH30406.02 FOETAL BOVINE SERUM 500ml HyClone SH30401.01 New Zealand Calf Serum 500ml HyClone SH30066.02 FetalClone  II 100ml HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml HyClone SH30118.02 Newborn Bovine Calf Serum, U.S. Origin 100ml HyClone SH30070.02 FETAL BOVINE SERUM 100ml HyClone SH30087.02 COSMIC CALF  SERUM 100ml HyClone SH30070.03E FETALBOVINESERUM CHARACTERIZSED 500ml 100ml HyClone SH30070.03 FETAL BOVINE SERUM 500ml HyClone SH30080.03 FetalClone  I 500ml HyClone SH30084.03 FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN 500ml HyClone SH30396.03 FETAL BOVINE SERUM CHARACTERIZED 500ml HyClone SV30087.02 南美源胎牛血清 500ml HyClone sv30087.01 南美源胎牛血清 100ml HyClone SH30071.03 FETAL BOVINE SERUM 500ml HyClone SH30071.02 FETAL BOVINE SERUM 100ml HyClone SH30080.02 FetalClone  I 100ml HyClone SH30070.02E FETAL BOVINE SERUM CHARACTERIZSED 100ml HyClone SH30079.02 FETAL BOVINE SERUM 100ml HyClone SH30068.02 CHARCOAL/DEXTRAN TREATED FBS，
活性炭滅活血清 100ml HyClone SH30068.03 CHARCOAL/DEXTRAN TREATED FBS，
活性炭滅活血清 500ml HyClone SH30066.03 FetalClone  II 500ml HyClone SH30109.03 HyClone FetalClone  III 500ml HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml HyClone SH30088.02 STANDARD FETAL BOVINE SERUM 100ml HyClone SH30079.03 FETAL BOVINE SERUM 500ml

 

 

第二部分  Gibco胎牛血清

 

 

品牌 貨號 名稱 規(guī)格 Gibco 12662-029 MSC QUALIFIED FBS 500ML 500ML Gibco 10438-026 FBS, HI 500ML 500ML  Gibco 10439-024 FBS, ES CELL QUAL. USDA APPD. 500ML 500ML Gibco 10099-141 FBS, AUSTRALIAN ORIGIN 500ML 500ML  Gibco 26400-044 FBS, DIALYZED, USA ORIGIN 500ML 500ML Gibco 26140-079 FBS QUALIFIED, USA ORIGIN 500ML 500ML Gibco 16141-079 FBS, ES CELL QUAL, USA ORIGIN 500ML 500ML Gibco 16140-071 FBS, HI 500ML 500ML Gibco 16000-044 FBS, CERT, USA ORIGIN 500ML 500ML Gibco C2027050   FBS, URUGUAYAN ORIGIN  500ML Gibco 12657-029 巴西胎牛 500ml