美國(guó)Seracare衣原體IgG(Chlamydia IgG)
廣州健侖生物科技有限公司
廣州健侖長(zhǎng)期供應(yīng)各種生物原料,主要代理品牌:美國(guó)Seracare、西班牙Certest、美國(guó)Fuller等等。
主要產(chǎn)品包括各種標(biāo)準(zhǔn)品、陽(yáng)性對(duì)照品、單克隆抗原抗體。
其中常見(jiàn)的有:弓形蟲病、西尼羅河病毒、類風(fēng)濕因子、瘧疾、麻疹、萊姆病、百日咳桿菌、大腸桿菌、鼠傷寒沙門氏菌、李斯特菌等陽(yáng)性對(duì)照品。
美國(guó)Seracare衣原體IgG(Chlamydia IgG)
我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。
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【Seracare產(chǎn)品介紹】
編號(hào)
英文名稱
中文名稱
JL-FA-01
Amebiasis (AME)
阿米巴病
JL-FA-02
Allergens, Rast scores
過(guò)敏原,放射性過(guò)敏原吸收實(shí)驗(yàn)。指對(duì)特定的人群引起免疫反應(yīng)或者過(guò)敏反應(yīng)的食品中的蛋白質(zhì)
JL-FA-03
Allergens, Rast scores negative
過(guò)敏原,放射性過(guò)敏原吸收實(shí)驗(yàn)陰性
JL-FA-04
Anti-cyclic citrullinated peptide Antibody (CCP) Arthritis
抗環(huán)瓜氨酸肽抗體
JL-FA-05
ASCA Saccharomyces Cerevi
人抗釀酒酵母抗體(ASCA)
JL-FA-06
Aspergillis
麴菌病
JL-FA-07
Beta 2 Glycoprotein
2糖蛋白
JL-FA-08
Beta 2 Glycoprotein IgM
2糖蛋白 IGM
JL-FA-09
Bordela Pertussis
百日咳桿菌
JL-FA-10
Bordela Pertussis IgM
百日咳桿菌 IGM
JL-FA-11
C-ANCA
C-抗中性粒細(xì)胞胞漿抗體(ANCA)
JL-FA-12
Cardiolipin
心肌磷脂
JL-FA-13
Cardiolipin IgA
心肌磷脂 IGA
JL-FA-14
Cardiolipin IgG
心肌磷脂 IGG
JL-FA-15
Cardiolipin IgM
心肌磷脂 IGM
JL-FA-16
Cerebral Spinal Fluid
腦脊髓液
JL-FA-17
Chagas
恰加斯病/南美錐蟲
JL-FA-18
Chlamydia
衣原體
JL-FA-19
Chlamydia IgA
衣原體IGA
JL-FA-20
Chlamydia IgG
衣原體IGG
JL-FA-21
Chlamydia IgM
衣原體IGM
JL-FA-22
Chlamydia Neg
衣原體陰性
JL-FA-23
Clotting Factor C3
凝固因子C3
JL-FA-24
Clotting Factor C4
凝固因子C4
JL-FA-25
Coccidiodes
球孢菌
JL-FA-26
Cytomegalovirus (CMV) Neg
巨細(xì)胞病毒抗體陰性
JL-FA-27
CMV IgG
巨細(xì)胞病毒 IGG陽(yáng)性
JL-FA-28
CMV IgM VCA
巨細(xì)胞病毒 IGM 陽(yáng)性
JL-FA-29
C-Reactive Protein (CRP)
C-反應(yīng)蛋白質(zhì)
JL-FA-30
Dengue Fever
登革熱
JL-FA-31
Dengue Fever IgM
登革熱 IGM
JL-FA-32
DS (Double Stranded) DNA
雙鏈脫氧核糖核酸
JL-FA-33
EBNA (Epstein-Barr nuclear antigen) IgG
EB病毒核抗原 IGG
JL-FA-34
EBNA (Epstein-Barr nuclear antigen) IgM
EB病毒核抗原 IGM
JL-FA-35
Epstein Barr Virus (EBV) Negative Plasma
EB病毒陰性血漿
JL-FA-36
Epstein Barr Virus (EBV) EA IgM
EB病毒早期抗原 IGM
JL-FA-37
Epstein Barr Virus (EBV) VCA IgM
EB病毒殼蛋白 IGM
JL-FA-38
Epstein Barr Virus (EBV) EA IgG
EB病毒早期抗原 IGG
JL-FA-39
EMA (Endomysial Antibodies)
肌內(nèi)膜
JL-FA-40
Gliadin
麩蛋白,麥醇溶蛋白,麥膠蛋白
JL-FA-41
Gliadin IgG
麥醇溶蛋白 IGG
JL-FA-42
Gliadin IgA
麥醇溶蛋白 IGA
JL-FA-43
Glomerular Basement Membrane (GBMA)
腎小球基底膜病
JL-FA-44
Helicobacter pylori IgA
幽門螺旋桿菌IGA
JL-FA-45
Helicobacter pylori IgG
幽門螺旋桿菌IGG
JL-FA-46
Helicobacter pylori IgM
幽門螺旋桿菌IGM
JL-FA-47
Helicobacter pylori Negative
幽門螺旋桿菌陰性
JL-FA-48
Helicobacter pylori Positive Plasma
幽門螺旋桿菌陰性血漿
JL-FA-49
Hepatitis A Virus (HAV) Pos. Plasma
甲型肝炎病毒陽(yáng)性血漿
JL-FA-50
Hepatitis A Virus (HAV) IgM
甲型肝炎病毒IGM
JL-FA-51
Hepatitis B Core (HBc) IgG
乙型肝炎病毒核心 IGG
JL-FA-52
Hepatitis B Core (HBc) IgM
乙型肝炎病毒核心 IGM
JL-FA-53
Anti Hbe (Antibody to HBV antigen)
乙肝抗體
JL-FA-54
Hepatitis Delta Virus
丁型肝炎病毒
JL-FA-55
HBeAg (HBV e antigen)
乙肝 E抗原
JL-FA-56
anti-HBs (HBV surface antibody)
乙肝表面抗體
JL-FA-57
Hepatitis B (HBsAg) "Chronic"
乙型肝炎(乙肝表面抗原) 慢性病
JL-FA-58
HBsAg (HBV surface antigen) Serum
乙肝表面抗原血清
JL-FA-59
HBsAg (AD)
乙肝表面抗原(AD)
JL-FA-60
HBsAg (AY)
乙肝表面抗原(AY)
JL-FA-61
HBV Positive Plasma
乙肝陽(yáng)性血漿
JL-FA-62
HBV DNA Plasma
乙肝DNA血漿
JL-FA-63
HBV DNA Serum
乙肝DNA血清
JL-FA-64
HBV DNA type A
A型 乙肝DNA
JL-FA-65
HBV DNA type B
B型 乙肝DNA
JL-FA-66
HBV DNA type C
C型 乙肝DNA
JL-FA-67
HBV DNA type D
D型 乙肝DNA
JL-FA-68
HBV DNA type E
E型 乙肝DNA
JL-FA-69
HBV DNA type F
F型 乙肝DNA
JL-FA-70
HBV Antibody HCV Antibody Plasma CO-INFECTED
乙肝和丙肝聯(lián)合感染血漿
JL-FA-71
HCV (Hepatitis C Virus) Antibody
丙型肝炎抗體
JL-FA-72
HCV Core Antigen Positive
丙肝核心抗原 陽(yáng)性
JL-FA-73
HCV RNA PLASMA Genotype 1
基因1型丙肝RNA 血漿
JL-FA-74
HCV RNA PLASMA Genotype 2
基因2型丙肝RNA 血漿
JL-FA-75
HCV RNA PLASMA Genotype 3
基因3型丙肝RNA 血漿
JL-FA-76
HCV RNA PLASMA Genotype 4
基因4型丙肝RNA 血漿
JL-FA-77
HCV RNA PLASMA Genotype 5
基因5型丙肝RNA 血漿
JL-FA-78
HCV RNA PLASMA Genotype 6
基因6型丙肝RNA 血漿
JL-FA-79
HCV Riba single band
丙肝免疫印跡單波段
JL-FA-80
HCV RIBA Pos. (multiple bands)
丙肝免疫印跡陽(yáng)性多波段
JL-FA-81
HCV Negative
丙肝陰性
JL-FA-82
HCV RNA Pos (quantitative)
丙肝RNA陽(yáng)性(定量)
JL-FA-83
Hepatitis E
戊型肝炎
JL-FA-84
Herpes Simplex Virus (HSV)1/2 Positive Plasma
單純性皰疹病毒1/2陽(yáng)性血漿
JL-FA-85
Herpes Simplex Virus (HSV) 1 Negative Plasma
單純性皰疹病毒1 陰性血漿
JL-FA-86
Herpes Simplex Virus (HSV) 1 IgG
單純性皰疹病毒1 IGG
JL-FA-87
Herpes Simplex Virus (HSV 1) IgM
單純性皰疹病毒1 IGM
JL-FA-88
Herpes Simplex Virus (HSV) 2 IgG
單純性皰疹病毒2 IGG
JL-FA-89
Herpes Simplex Virus (HSV) 2 IgM
單純性皰疹病毒2 IGG
JL-FA-90
Histone
組蛋白
JL-FA-91
Human Anti Mouse Ab (HAMA)
人抗鼠抗體
JL-FA-92
Human immunodeficiency virus (HIV) 1 Neg
HIV I 陰性
JL-FA-93
anti Human immunodeficiency virus (HIV) 1 Plasma
抗HIV I 血漿
JL-FA-94
anti Human immunodeficiency virus (HIV) 1 Serum
抗HIV I 血清
JL-FA-95
anti Human immunodeficiency virus (HIV) 2 Western Blot Tested
抗HIV 2 免疫印跡
JL-FA-96
anti Human immunodeficiency virus (HIV) 1/2 2 HIV (+)
抗HIV 1/2 2 HIV陽(yáng)性
JL-FA-97
Human immunodeficiency virus (HIV) Ag
HIV抗原
JL-FA-98
HIV RNA (quantitative) Plasma
HIV RNA 定量血漿
JL-FA-99
HIV RNA (quantitative) Serum
HIV RNA 定量血清
JL-FA-100
HIV1 Subtype A
HIV1 亞型A
JL-FA-101
HIV1 Subtype B
HIV1 亞型B
JL-FA-102
HIV1 Subtype C
HIV1 亞型C
JL-FA-103
HIV1 Subtype D
HIV1 亞型D
JL-FA-104
HIV1 Subtype E
HIV1 亞型E
JL-FA-105
HIV1 Subtype F
HIV1 亞型F
JL-FA-106
HIV1 Subtype G
HIV1 亞型G
JL-FA-107
HIV1 Subtype H
HIV1 亞型H
JL-FA-108
HIV1 Subtype J
HIV1 亞型J
JL-FA-109
HIV1 Subtype K
HIV1 亞型K
JL-FA-110
HIV1 Group O
HIV1 亞型O
JL-FA-111
Human immunodeficiency virus (HIV) 2 Antibody Plasma
HIV 2 抗體血漿
JL-FA-112
Human immunodeficiency virus (HIV) 2 Antibody Serum
HIV 2 抗體血清
JL-FA-113
HPV (Human Papiloma Virus) Negative
人乳狀瘤病毒HPV陰性
JL-FA-114
HPV (Human Papiloma Virus) Positive
人乳狀瘤病毒HPV陽(yáng)性
JL-FA-115
Human immunodeficiency virus (HIV) Antibody HCV Antibody Plasma COINFECTED
HIV 抗體 HCV
JL-FA-116
Human T-cell Lymphotropic Virus (HTLV) I/II
人嗜T淋巴細(xì)胞病毒(HTLV) I/II
JL-FA-117
Human T-cell Lymphotropic Virus (HTLV) I
人嗜T淋巴細(xì)胞病毒(HTLV) I
JL-FA-118
Human T-cell Lymphotropic Virus (HTLV) II
人嗜T淋巴細(xì)胞病毒(HTLV) II
JL-FA-119
Jo-1
多發(fā)性肌炎抗原JO-1
JL-FA-120
IgE 5,000 Ku/L
IgE 5,000 Ku/L
JL-FA-121
Legionella
軍團(tuán)桿菌屬
JL-FA-122
Leptospira
軍團(tuán)桿菌屬
JL-FA-123
Lyme Disease
萊姆(氏)病:蜱傳播的全身性疾病,常在夏季發(fā)生
JL-FA-124
Lyme IgG
萊姆(氏)病 IGG
JL-FA-125
Lyme IgM
萊姆(氏)病 IGM
JL-FA-126
Lyme Disease Neg
萊姆(氏)病 陰性
JL-FA-127
Malaria
瘧疾
JL-FA-128
Mononucleosis (infectious)
單核細(xì)胞增多癥(有傳染性的)
JL-FA-129
Mononucleosis Negative
單核細(xì)胞增多癥陰性
JL-FA-130
Measles Negative
麻疹 陰性
JL-FA-131
Measles IgG
麻疹 IGG
JL-FA-132
Measles IgM
麻疹 IGM
JL-FA-133
Microsomal Anti-thyroid peroxidase antibody (TPO) Positive Plasma Standard Titer (typically 1,000-3,000 IU/mL)
微粒體抗甲狀腺過(guò)氧化物酶抗體
JL-FA-134
Microsomal Anti-thyroid peroxidase antibody (TPO) Negative Plasma
微粒體抗甲狀腺過(guò)氧化物酶抗體
JL-FA-135
Anti-mitochondrial antibody (AMA)
抗線粒體抗體
JL-FA-136
Multiple Sclerosis
多發(fā)性硬化癥
JL-FA-137
Mumps IgG
流行性腮腺炎 IGG
JL-FA-138
Mumps Ab IgM
流行性腮腺炎抗體 IGM
JL-FA-139
Mumps Antibody Negative Plasma
流行性腮腺炎抗體陰性血漿
JL-FA-140
Mumps Antibody Negative Serum
流行性腮腺炎抗體陰性血清
JL-FA-141
Myeloma Plasma
骨髓瘤血漿
JL-FA-142
Myeloma IgA
骨髓瘤IGA
JL-FA-143
Myeloma IgE
骨髓瘤IGE
JL-FA-144
Myeloma IgG
骨髓瘤IGG
JL-FA-145
Myeloma IgM
骨髓瘤IGM
JL-FA-146
Mycoplasma
支原體
JL-FA-147
Mycoplasma Negative
支原體陰性
JL-FA-148
Mycoplasma IgG
支原體IGG
JL-FA-149
Mycoplasma IgM
支原體IGM
JL-FA-150
Mycoplasma PCR
支原體PCR
JL-FA-151
Normal Human Plasma
正常人血漿
JL-FA-152
Normal Human Serum
正常人血清
JL-FA-153
Nuclear Antibody Centromere
核抗體著絲粒
JL-FA-154
Nuclear Antibody, Speckled ANA
核抗體,斑點(diǎn)抗核抗體
JL-FA-155
Nuclear Antibody, Nucleolar ANA
核抗體,核仁抗核抗體
JL-FA-156
Nuclear Antibody, Homogeneous ANA
核抗體,同質(zhì)抗核抗體
JL-FA-157
Nuclear Antiobody, Speckled. (ANA) Negative
核抗體,斑點(diǎn)。抗核抗體陰性
JL-FA-158
P-ANCA (associated neutrophil cytoplasmic antibodies)
相關(guān)的嗜中性粒細(xì)胞胞漿抗體
JL-FA-159
Parietal Cell Antibody (PCA)
胃)壁細(xì)胞抗體
JL-FA-160
Parvo positive plasma
細(xì)小病毒陽(yáng)性血漿
JL-FA-161
Parvo IgM
細(xì)小病毒 IGM
JL-FA-162
Parvo IgG
細(xì)小病毒 IGG
JL-FA-163
Parvo Negative Plasma
細(xì)小病毒陰性血漿
JL-FA-164
Parvo DNA positive
細(xì)小病毒 DNA 陽(yáng)性
JL-FA-165
Phospholipid Positive Plasma
磷脂陽(yáng)性血漿
JL-FA-166
Prothrombin
凝血酶原,凝血因子
JL-FA-167
Rheumatoid Factor (RF) 1000 IU/mL
類風(fēng)濕因子 1000 IU/mL
JL-FA-168
Rheumatoid Factor (RF) 1001-2000 IU/mL
類風(fēng)濕因子1001-2000 IU/mL
JL-FA-169
Rheumatoid Factor (RF) 2001-4000 IU/mL
類風(fēng)濕因子 2001-4000 IU/mL
JL-FA-170
Rheumatoid Factor (RF) 4001-5000 IU/mL
類風(fēng)濕因子 4001-5000 IU/mL
JL-FA-171
Rheumatoid Factor (RF) 5000 IU/mL
類風(fēng)濕因子 5000 IU/mL
JL-FA-172
Ribonucleoprotein (RNP) Positive
核糖核蛋白陽(yáng)性
JL-FA-173
Rubella Chimeric
風(fēng)疹
JL-FA-174
Rubella Negative
風(fēng)疹陰性
JL-FA-175
Rubella IgG
風(fēng)疹I(lǐng)GG
JL-FA-176
Rubella IgM
風(fēng)疹I(lǐng)GM
JL-FA-177
Rubeola Negative Plasma
風(fēng)疹陰性血漿
JL-FA-178
Rubeola IgG
風(fēng)疹I(lǐng)GG
JL-FA-179
Scleroderma (Scl-70) Pos
膠原沉著病,硬皮病,硬皮癥 陽(yáng)性
JL-FA-180
Scleroderma (Scl-70) Negative
硬皮病陰性
JL-FA-181
Sickle Cell Fresh Whole Blood
鐮刀形紅細(xì)胞新鮮全血
JL-FA-182
Smith (SM)
抗Smith抗體陽(yáng)性血清(SLE的特征性抗體)
JL-FA-183
SMITH RNP
抗RNP抗體陽(yáng)性血清(SLE的特征性抗體)
JL-FA-184
Smooth Muscle (ASMA)
抗平滑肌抗體陽(yáng)性血清
JL-FA-185
Sjogren syndrome antigen A (SSA) Positive
舍格倫綜合征或干燥綜合征抗原A 陽(yáng)性
JL-FA-186
Sjogren syndrome antigen B (SSB) Positive
舍格倫綜合征抗原B 陽(yáng)性
JL-FA-187
Sjogren syndrome antigen B (SSB) Negative
舍格倫綜合征抗原B陰性
JL-FA-188
Streptolysin O Ab (ASO)
鏈球菌溶血素O抗體
JL-FA-189
Syphilis (RPR - Rapid Plasma Reagin) Positive Plasma
梅毒(梅毒-快速血漿反應(yīng))陽(yáng)性血漿
JL-FA-190
Syphilis (RPR - Rapid Plasma Reagin) Negative Plasma
梅毒(梅毒-快速血漿反應(yīng))陰性血漿
JL-FA-191
Syphilis/ATA/T. pallidum IgG
梅毒ATA/T,蒼白球IGG
JL-FA-192
Syphilis/ATA/T. pallidum IgM
梅毒ATA/T,蒼白球IGM
JL-FA-193
Systemic Lupus Erythematosus (SLE) Positive
全身性紅斑狼瘡陽(yáng)性
JL-FA-194
Systemic Lupus Erythematosus (SLE) Negative
全身性紅斑狼瘡陰性
JL-FA-195
TG/TPO Positive (Standard Titer 1,000 - 3000 IU/mL)
甲狀腺球蛋白/甲狀腺過(guò)氧化物酶陽(yáng)性
JL-FA-196
TG/TPO Negative
甲狀腺球蛋白/甲狀腺過(guò)氧化物酶陰性
JL-FA-197
TTG (Tissue Transglutaminase)
組織轉(zhuǎn)谷氨酰胺酶
JL-FA-198
TTG (Tissue Transglutaminase) IgA
組織轉(zhuǎn)谷氨酰胺酶 IGA
JL-FA-199
ToRCH (Toxo, Rubella, CMV, HSV) Positive
優(yōu)生優(yōu)育(弓形蟲,風(fēng)疹,巨細(xì)胞,單胞)陽(yáng)性
JL-FA-200
ToRCH (Toxo, Rubella, CMV, HSV) Negative
優(yōu)生優(yōu)育(弓形蟲,風(fēng)疹,巨細(xì)胞,單胞)陰性
JL-FA-201
Toxoplasmosis (Toxo)
弓形蟲病
JL-FA-202
Toxoplasmosis (Toxo) IgG
弓形蟲病IGG
JL-FA-203
Toxoplasmosis (Toxo) IgM
弓形蟲病IGM
JL-FA-204
Thyroglobulin (TG) Positive Plasma
甲狀腺球蛋白陽(yáng)性血漿
JL-FA-205
Thyroglobulin (TG) Negative
甲狀腺球蛋白陰性
JL-FA-206
Varicella-Zoster Virus (VZV) Negative
水痘-帶狀皰疹病毒陰性
JL-FA-207
Varicella-Zoster Virus (VZV) IgG
水痘-帶狀皰疹病毒IGG
JL-FA-208
Varicella-Zoster Virus (VZV) IgM
水痘-帶狀皰疹病毒IGM
JL-FA-209
West Nile Virus (WNV)
西尼羅河腦炎病毒
JL-FA-210
West Nile Virus (WNV) IgM
西尼羅河腦炎病毒IGM
美國(guó)Seracare衣原體IgG(Chlamydia IgG)
美國(guó)斯隆-凱特琳研究所等處的研究人員創(chuàng)建了一種多能干細(xì)胞基因組編輯平臺(tái):iCRISPR,這一平臺(tái)能快速,高效的敲除干細(xì)胞中的基因,而且還能在干細(xì)胞分化過(guò)程中,進(jìn)行階段特異性的基因敲除,這將在人類疾病復(fù)雜病理研究中大放異彩。相關(guān)文章發(fā)表于2014年6月12日的《Cell Stem Cell》雜志上。
人體多能干細(xì)胞(hPSCs)不僅能被用于臨床的再生研究應(yīng)用中,而且也能作為解析復(fù)雜性狀和特征的獨(dú)特平臺(tái),闡明其背后的基因和分子途徑。為了實(shí)現(xiàn)這一目的,科學(xué)家們開(kāi)發(fā)了多種遺傳操控方法,但是這些方法依然存在各種問(wèn)題,我們需要快速,具有可操控性的生物學(xué)手段。
在這篇文章中,研究人員就利用CRISPR和TALEN,這兩種備受關(guān)注的基因組編輯技術(shù),研發(fā)出了一種人類多能干細(xì)胞基因組編輯平臺(tái)。研究人員將這一平臺(tái)稱為iCRISPR。
iCRISPR能用于基因功能喪失研究中,快速,高效的敲除人體多能干細(xì)胞中的等位基因,也可以針對(duì)一些精確的疾病模型,通過(guò)特定的核苷酸變換,進(jìn)行多能干細(xì)胞純合體敲除。
通過(guò)進(jìn)一步實(shí)驗(yàn),研究人員驗(yàn)證了雙重和三重基因敲除hPSC細(xì)胞系一步法的有效性,同時(shí)也證明了在多能干細(xì)胞分化過(guò)程中能進(jìn)行階段特異性誘導(dǎo)基因敲除,這對(duì)于發(fā)育生物學(xué)研究來(lái)說(shuō)意義重大。
由此研究人員指出,iCRISPR平臺(tái)尤其適合用于解析人類疾病研究中的復(fù)雜遺傳相互作用,以及多效性基因功能,這將有助于進(jìn)行人體多能干細(xì)胞高通量遺傳分析。
除了這項(xiàng)研究之外,去年來(lái)自加州大學(xué)舊金山分校的研究人員提出了一個(gè)相似的名稱:CRISPRi,他們發(fā)現(xiàn)當(dāng)缺失核酸內(nèi)切酶活性的Cas9與一種導(dǎo)向RNA共表達(dá)時(shí)候,會(huì)產(chǎn)生一種DNA識(shí)別復(fù)合物,這種復(fù)合物能特異性干擾轉(zhuǎn)錄延伸,RNA聚合酶結(jié)合,或轉(zhuǎn)錄因子結(jié)合。
由此研究人員研發(fā)出了這種CRISPRi系統(tǒng),這一系統(tǒng)能有效抑制大腸桿菌中靶向基因的表達(dá),并且不會(huì)出現(xiàn)脫靶效應(yīng)。而且利用CRISPRi,還可以同時(shí)抑制多個(gè)靶基因,這種作用也是可逆。研究人員還證明,該系統(tǒng)也適用于哺乳動(dòng)物細(xì)胞中的基因表達(dá)抑制。
加州大學(xué)圣迭戈分校(University of California, San Diego)醫(yī)學(xué)院研究人員針對(duì)自閉癥研究出一套比較新銳的理論,并對(duì)其進(jìn)行了實(shí)際測(cè)試。研究結(jié)果顯示,自閉癥實(shí)際上是由異常的細(xì)胞通訊導(dǎo)致的,使用問(wèn)世已逾百年的治療嗜睡癥的藥物,可以恢復(fù)患有自閉癥的老鼠體內(nèi)的細(xì)胞活動(dòng),消除老鼠的神經(jīng)失調(diào)癥狀。參與實(shí)驗(yàn)的老鼠年齡相當(dāng)于人類30歲左右。相關(guān)研究成果在2014年6月17日的在線刊物《平移精神病學(xué)(Translational Psychiatry)》中發(fā)表。
美國(guó)Seracare衣原體IgA(Chlamydia IgA)
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【公司名稱】 廣州健侖生物科技有限公司
【】 楊永漢
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【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-3室
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Researchers at the U.S.-based Sloan-Caitlin Institute have created iCRISPR, a pluripotent stem cell genomics editing platform that rapidly and efficiently knocks out genes in stem cells and helps them differentiate stem cells during stem cell differentiation, Performing stage-specific knockouts will shine in the complex pathology of human disease. The article appeared in the June 12, 2014 issue of Cell Stem Cell.
Not only can human pluripotent stem cells (hPSCs) be used in clinical regenerative research applications, but also as a unique platform for analyzing complex traits and traits, elucidating the underlying genetic and molecular pathways. To achieve this goal, scientists have developed a variety of genetic manipulation methods, but there are still a variety of problems with these methods, and we need fast, manipulative biological tools.
In this article, researchers developed a human pluripotent stem cell genome editing platform using two well-regarded genome editing technologies, CRISPR and TALEN. The researchers call this platform iCRISPR.
iCRISPR can be used in gene loss studies to rapidly and efficiently knock out alleles in pluripotent human pluripotent stem cells and to target specific models of disease through specific nucleotide transforms for pluripotent stem cell homozygous knocking except.
Through further experiments, the researchers validated the one-step efficacy of double and triple knockout hPSC cell lines, and also demonstrated that stage-specific induction of gene knockdown during pluripotent stem cell differentiation can be performed in developmental biology Meaningful.
As a result, the researchers noted that the iCRISPR platform is particularly well-suited for the analysis of complex genetic interactions in human disease research and pleiotropic gene functions that will facilitate high-throughput genetic analysis of human pluripotent stem cells.
In addition to the study, researchers from the University of California, San Francisco, last year proposed a similar name: CRISPRi, who found that a DNA lacking endonuclease co-expressed with a directing RNA produced a DNA Recognition complexes that specifically interfere with transcriptional elongation, RNA polymerase binding, or transcription factor binding.
As a result, the researchers developed the CRISPRi system, which effectively inhibits the expression of the targeted genes in E. coli and does not show off-target effects. And using CRISPRi, you can also inhibit multiple target genes, this effect is also reversible. The researchers also demonstrated that the system is also suitable for gene expression inhibition in mammalian cells.
Researchers at the University of California, San Diego Medical School developed a set of cutting-edge theories of autism and conducted practical tests. The results show that autism is actually caused by abnormal cell communication, the use of more than a century has come out of the treatment of narcolepsy drugs, can restore autism in mice with cell activity in vivo, to eliminate symptoms of neurological disorders in mice . The age of the mice involved in the experiment is about 30 years old. Relevant research results are published in the June 17, 2014 online translation "Translational Psychiatry."