歐羅拉差異裂解法,DNA前處理工作站,混合精斑前處理工作站

differential digestion workstation

在法醫(yī)學(xué)實(shí)踐中，混合斑檢材以精陰混合斑最為常見(jiàn)，即精液與陰道分泌液的混合物。

Forensic sexual assault cases with mixed samples of semen and epithelial cells are very common.

對(duì)于此類(lèi)檢材，都必須采用差異裂解法進(jìn)行精子細(xì)胞分離。

For such samples, differential digestion method must be used to separate sperm cells.

隨著技術(shù)改進(jìn)，結(jié)合脫氧核苷酸酶Dnase Ⅰ和改良的堿性裂解液，以及自動(dòng)化液體工作站平臺(tái)，即可輕松快速?gòu)暮芯叩幕旌弦褐蝎@得精子DNA。

With the improvement of technology, sperm DNA can be easily and quickly obtained from the sample mixture by combining deoxynucleotidase Dnase I with improved alkaline lysate and automated liquid workstation platform.

應(yīng)用： 

Application: 

食品安全檢測(cè) 

_Food safety testing 

血液/血清中維生素D萃取 

Extraction of Vitamin D from Blood/Serum 

公安藥物實(shí)驗(yàn)室中濫用檢測(cè) 

_Detection of Toxic Substance Abuse in Public Security Drug Laboratory 

血液中促進(jìn)生長(zhǎng)劑的檢測(cè) 

_Detection of growth-promoting agents in blood 

水產(chǎn)品中禁用藥物，如孔雀石綠 

_Prohibited drugs in aquatic products, such as malachite green 

藥物研發(fā)化合物純化 

_Purification of Drug R&D Compounds 

尿液中異黃酮分離 

Detection of Isoflavonoides from urine samples 

海產(chǎn)品中的黃曲霉素檢測(cè) 

_Detection of Aflatoxin in seafood 

非揮發(fā)或半揮發(fā)分析化合物處理 

_Treatment of Non-volatile or Semi-volatile Analytical Compounds 

食品中氯霉素 

_Chloramphenicol in Food

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Applications 

Genomics

·   Automated Isolation of Genomic DNA using the MACHEREY- NAGEL NucleoMag Plant kit by Aurora Biomed’s VERSA 1100

·   Automated Isolation of Genomic DNA using the MACHEREY-NAGEL NucleoMag Blood 200 L kit by Aurora Biomed’s VERSA 1100

·   采用性犯罪試劑盒差異消化方法在VERSA 1100自動(dòng)化應(yīng)用

·   VERSA 1100 GENE在下一代測(cè)序（NGS）文庫(kù)制備自動(dòng)化的可行性驗(yàn)證

·   全血樣品中核酸提取應(yīng)用報(bào)告

·   植物樣品中核酸提取應(yīng)用報(bào)告

·   Automation of DNA Extraction

·   PCR Setup

·   Automation of Reverse Transcriptase PCR

·   Automation of Real time PCR

·   Automation of RNA Sequencing

·   Automation of Next Generation Sequencing

·   Automation of DNA Microarray

·   Automation of Miniprep

·   Automation of Sanger Sequencing

·   Automation of On-Slide (Amplislide) PCR Setup using VERSA 110 PCR Setup Workstation

·   Food Safety Monitoring using VERSA 110 NAP Workstation

·   Hot-Start PCR using VERSA 110 PCR Workstation

·   DNA Isolation from Saliva (Invitek Forensic DNA Isolation Kit)

·   Nucleic Acid Prep for Avian Flu Viral RNA

·    -Actin and Whole Genome Amplification (Sigma & Promega kits)

·   Genomic DNA Isolation from Blood (Promega)

·   Automation of Molecular Pathology Applications on the VERSA 10 PCR Setup Workstation

·   Automated System for High Throughput PCR Setup

Extraction

·   高通量固相萃取&氣相色譜-質(zhì)譜聯(lián)用方法定量檢測(cè)吸毒者尿液中甲基苯丙胺和苯丙胺

·   HTS Flux Assay Automation

·   Validation of Automated Liquid Liquid Extraction of 25-hydroxy vitamin D

·   Automation of Sample Preparation and Introduction into NMR Tubes

·   Liquid Liquid Extraction of -carotene

·   Automation of Protein Purification

General Liquid Handling

·   High-Density Peptide Array Printing

·   Specimen Staining for TEM (Array printing)

·   Automated Slide-based Assay Setup using VERSA 110 Workstation

·   VERSA Spotter Workstation for Solid-Phase Peptide Synthesis

·   Automated Protein Crystallography Plate Setup using VERSA 110

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創(chuàng)新點(diǎn)：

儀器針對(duì)法醫(yī)學(xué)實(shí)踐中的混合檢材，尤其是精陰混合斑，采用特殊的差異裂解法進(jìn)行精子細(xì)胞分離。 現(xiàn)混合精斑DNA前處理工作站，將差異裂解法在液體工作站中特色設(shè)計(jì)為自動(dòng)化，結(jié)合脫氧核苷酸酶DnaseⅠ和改良的堿性裂解液，，即可輕松快速?gòu)暮芯叩幕旌弦褐蝎@得精子DNA。 現(xiàn)特色模塊如實(shí)際冷槽，對(duì)緩沖液、生物酶、試劑等低溫保存，提高提取效率。 典型案例： 加利福尼亞州奧克蘭警察局采用我司VERSA1100差異裂解法進(jìn)行法醫(yī)分析，并發(fā)表了論文證明了他們的成功。論文鏈接https://www.ncjrs.gov/pdffiles1/nij/grants/242773.pdf

歐羅拉混合精斑DNA前處理工作站（差異裂解法）VERSA1100