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Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1緊密連接蛋白抗體產(chǎn)品信息：Product name：Anti-ZO1 tight junction protein antibodySee all ZO1 tight junction protein primary antibodies ...Description

Rabbit polyclonal to ZO1 tight junction proteinSpecificityab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms. The protein is abundant in endothelial cells and the highly specialized epithelial junctions. Although we have an image for IHC-Fr, customers report variable results in this application. If you would like to use in IHC-Fr then we suggest using ethanol instead of acetone. Although we have references for IHC-P, customers report variable results in this application. IHC-Fr and IHC-P are therefore not covered by our Abpromise guarantee.Tested applicationsICC/IF, Flow Cyt, IHC (PFA fixed), WB, IHC-P more detailsSpecies reactivity

Reacts with: Mouse, Chicken, Dog, Human, PigImmunogen

Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein

PropertiesFormLiquidStorage instructionsShipped at 4 C. Upon delivery aliquot and store at -20 C. Avoid freeze / thaw cycles.Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS

Concentration 50 g at 0.1 mg/mlPurification notes0.2 m filtered.Clonality PolyclonalIsotypeIgGResearch AreasTags Cell MarkersSubcellular MarkersPlasma Membrane

Applications

Our Abpromise guarantee covers the use of ab59720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.ApplicationNotesICC/IFICC/IF: Use a concentration of 1 g/ml.Flow CytFlow Cyt: 1/50.IHC (PFA fixed)IHC (PFA fixed): Use at an assay dependent concentration. PubMed: 21526120WBWB: 1/50. Predicted molecular weight: 194 kDa.IHC-PIHC-P: Use at an assay dependent concentration.

TargetFunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain.DomainThe second PDZ domain mediates interaction with GJA12.Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ.Cellular localizationCell membrane. Cell junction tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.Information by UniProt

Database linksEntrez Gene: 7082 HumanEntrez Gene: 21872 MouseOmim: 601009 HumanSwissProt: Q07157 HumanSwissProt: P39447 MouseUnigene: 510833 HumanUnigene: 4342 Mouse

Alternative namesTight junction protein 1 antibodyTight junction protein ZO-1 antibodyTight junction protein ZO1 antibody

Anti-ZO1 tight junction protein antibody images

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)

ab59720 staining ZO1 in Caco-2 cells treated with ( )-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of ( )-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37 C for 1 hour in media containing different concentrations of ab141122 (( )-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 g/ml) was performed overnight at 4 C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunocytochemistry/ Immunofluorescence - ZO1 tight junction protein antibody (ab59720)

ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1 g/ml) overnight at +4 C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 M.

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25 C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4 C. An AlexaFluor 488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)Image from Kokkinopoulos I et al., PLoS One. 2011 Apr 22;6(4):e18921. Fig 6.; doi:10.1371/journal.pone.0018921; April 22, 2011, PLoS ONE 6(4): e18921.

Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720.

Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor -conjugated anti-rabbit IgG was used as the secondary antibody.

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  Abcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，專(zhuān)門(mén)生產(chǎn)和分銷(xiāo)研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷(xiāo)售質(zhì)量高的抗體，并提供zui全面、誠(chéng)實(shí)、時(shí)新的說(shuō)明書(shū)，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線(xiàn)目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予全球百多個(gè)國(guó)家。Abcam于2005年11月在倫敦證券交易所上市，在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。

  Abcam 一直致力加強(qiáng)產(chǎn)品線(xiàn)，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購(gòu)了美國(guó)的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購(gòu)了英國(guó)的Ascent Scientific 公司，開(kāi)展了生化試劑的供應(yīng)。在2012年，Abcam并購(gòu)了美國(guó)的Epitomics 公司，成為一家有領(lǐng)導(dǎo)地位的RabMAbs 供應(yīng)商。

  Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線(xiàn)目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來(lái)使客戶(hù)獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來(lái)指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策，在我們的上會(huì)發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。

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