Antibody against posttranscripitional modifications of RNAsFact Sheet Cat. No. 202 002, 202 003

Polyclonal rabbit antibodyCat. No. 202 002
crude antiserum200 ?l antiserum, lyophilized. For reconstitution add 200 ?l H2O, then aliquot and store at -20 C until use.Cat. No. 202 003
purified antibody50 ?g specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 ?l H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 C until use.ApplicationsWB: no
IP: yes (see remarks)
ICC: not tested yet
IHC: not tested yetImmunogenN6-methyladenosine fused to BSA.ReactivityHuman, rat, mouse, Xenopus laevis, eukaryotes, prokaryotes.SpecificitySpecific for N6-metyladenosine (m6A).RemarksIP: Human extracts or extracts from Xenopus laevis. Standard protocol provided with the product.

Antibody against posttranscripitional modifications of RNAsReferences SySy m6A antibodies

Xiao R Moore DD (2011). DamIP: using mutant DNA adenine methyltransferase to study DNA-protein interactions in vivo. Current Protocols in Molecular Biology 21.21.1-10. affinity purified antibody; IP

Moore CL, Skolnik-David H Sharp PA (1988). Sedimentation analysis of polyadenylation-specific complexes. Molecular and Cellular Biology 8: 226-33. antiserum; IP

Bringmann P L hrmann R (1987). Antibodies specific for N6-methyladenosine react with intact snRNPs U2 and U4/U6. FEBS Letter 213: 309-15.