聚乙烯亞胺(細胞培養(yǎng))(Polyethyleneimine;PEI;Epomine)
將外源基因?qū)胝婧思毎欠肿由飳W研究中的一項重要技術(shù)。轉(zhuǎn)基因載體主要分為病毒載體系統(tǒng)和非病毒載體系統(tǒng)。病毒載體系統(tǒng)轉(zhuǎn)染效率高,是體內(nèi)基因治療的主要工具,但安全性存在隱患,且有免疫原性,體內(nèi)不能反復應用,制備較復雜,而且所能裝載的外源DNA大小有限,使其應用受到很大限制。非病毒載體是病毒載體的重要補充途徑,一般認為安全性高,載體一般無免疫原性,而且是基于質(zhì)粒的轉(zhuǎn)染,易于組裝[1-2]。目前已有的非病毒型載體介導基因轉(zhuǎn)染的方法有裸DNA注射、磷酸鈣介導、電轉(zhuǎn)移法、陽離子脂質(zhì)體及陽離子聚合物介導等。1995年,Boussif首次報道了聚乙烯亞胺(細胞培養(yǎng))(polyethylenimine,PEI)可作為非病毒載體,其后,聚乙烯亞胺成為陽離子多聚物研究zui快的領(lǐng)域。為了研究PEI基因轉(zhuǎn)染試劑的轉(zhuǎn)染效果,本文通過聚乙烯亞胺(細胞培養(yǎng))PEI轉(zhuǎn)染表達綠色熒光蛋白的pEGFP質(zhì)粒到293T細胞,以觀察其效果,并測定了多種因素對轉(zhuǎn)染效率的影響。
CAS Number: 9002-98-6
訂購信息:
貨號
產(chǎn)品名稱
規(guī)格
25448-500
Polyethyleneimine, branched (50% soln. in water, MW 750,000)
500g
25414-2
Polyethyleneimine, Linear (MW ~100,000)
2g
25439-2
Polyethylenimine "Max", (Mw 160,000) - High Potency Linear PEI
2g
24885-2
Polyethylenimine "Max"?, (Mw 4,000*) - High Potency Linear PEI (Equivalent to Mw ~2,500 in Free Base Form) Polyethylenimine
2g
24765-2
Polyethylenimine "Max"?, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)
2g
00618-500
Polyethylenimine, branched (30% soln. in water, MW 70,000)
500g
06088-100
Polyethylenimine, branched (MW 1,200)
100g
06088-500
Polyethylenimine, branched (MW 1,200)
500g
06089-100
Polyethylenimine, branched (MW 1,800)
100g
06089-500
Polyethylenimine, branched (MW 1,800)
500g
19850-100
Polyethylenimine, branched (MW 10,000)
100g
19850-500
Polyethylenimine, branched (MW 10,000)
500g
06090-100
Polyethylenimine, branched (MW 50,000-100,000)
100g
06090-500
Polyethylenimine, branched (MW 50,000-100,000)
500g
02371-100
Polyethylenimine, branched (MW 600)
100g
02371-500
Polyethylenimine, branched (MW 600)
500g
21903-10
Polyethylenimine, branched, permethylated, permethobromide
10g
24313-2
Polyethylenimine, Linear (MW 2,500)
2g
23966-2
Polyethylenimine, Linear (MW 25,000)
2g
24314-2
Polyethylenimine, Linear (MW 250,000)
2g